Isolation, screening, characterization, and identification of alkaline protease-producing bacteria from leather industry effluent

Table 3 Morphological and biochemical characterization of isolates

Characteristic	Bacterial isolates
Characteristic	ML5	ML12	MS12
Morphological features
Color	Creamy red	White	Creamy red
Shape	Rod	Rod	Long Rod
Surface	Smooth	Smooth	Smooth
Opacity	Opaque	Opaque	Opaque
Gram staining	Positive	Positive	Positive
Motility	Motile	Motile	Motile
Endospore staining	Positive	Positive	Positive
Biochemical characteristic
Sucrose hydrolysis	Positive	Positive	Positive
Lactose hydrolysis	Negative	Negative	Positive
Glucose hydrolysis	Positive	Positive	Positive
Fructose hydrolysis	Positive	Positive	Negative
Mannitol hydrolysis	Positive	Negative	Positive
Starch hydrolysis	Positive	Positive	Positive
Galactose hydrolysis	Positive	Positive	Positive
Indole test	Negative	Negative	Negative
Methyl red test	Negative	Positive	Positive
Urease test	Negative	Negative	Positive
Voges-Proskauer test	Positive	Negative	Positive
Casein hydrolysis	Positive	Positive	Positive
Citrate utilization test	Positive	Negative	Negative
Gelatin hydrolysis	Positive	Positive	Positive
Catalase test	Positive	Positive	Positive
Similarity of bacteria	Bacillus sp.	Bacillus sp.	Bacillus sp.

ISSN: 1869-2044