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Table 1 Total particles, prokaryotes and virus-like particles counts on 0.2- and 0.02-μm pore size membranes by epifluorescence microscopy

From: Proportion of prokaryotes enumerated as viruses by epifluorescence microscopy

  Sampling station
I2 I6
Pore size membranes Particles type Number (average ± SD L−1) % Number (average ± SD L−1) %
0.2 μm Total (DAPI stain) 4.6 ± 0.3 × 109 100 3.9 ± 0.2 × 109 100
Eubacteria (FISH Eub338 probe stain) 3.2 ± 0.3 × 109 70 2.4 ± 0.4 × 109 61
Archaea (FISH Arc915 probe stain) 3.0 ± 0.1 × 108 7 3.9 ± 0.04 × 108 10
Non-probe labelled (DAPI stain-probes stain) 1.1 ± 0.2 × 109 23 1.2 ± 0.2 × 109 29
0.02 μm Total (DAPI stain) 1.2 ± 0.06 × 1010 100 1.0 ± 0.09 × 1010 100
Eubacteria (FISH Eub338 probe stain) 2.6 ± 0.6 × 109 22 7.5 ± 0.2 × 109 7
Archaea (FISH Arc915 probe stain) 7.8 ± 0.3 × 108 6 6.1 ± 0.17 × 108 6
Viruses (DAPI stain-probes stain) 8.5 ± 0.6 × 109 72 9.0 ± 0.8 × 109 87
  1. Values represent the mean of three sub-samples. For each sub-sample, three replicates were counted